新生儿轮状病毒无症状感染的聚合酶链反应研究

Detection of Rotavirus from Asymptomtic Neonatal infants infection by Polymerase Chain Reaction

摘要:

选用与 A 组人类轮状病毒(Human Romvirus,HRV)编码 VP7蛋白的第9基因(或8)两个保守序列互补的引物,建立检测 A 组 HRV dsRNA 的聚合酶链反应技术,扩增产物片段约342bp。经逆转录—聚合酶链反应(RT—PCT)检测18例无腹泻症状新生儿89份粪便标本,并与聚丙烯酰胺凝胶电泳(PAGE)方法进行比较,结果前者检出 HRV 核酸54份(60.7%),而后者则检出35份(39.3%),PCR 结果可能比较接近新生儿排泌轮状病毒的实际情况。

Abstract:

The primers selected in this study are complementary to the common conserved sequences of gene 9 (or 8) a- mong A group four rotavirus serotypes.The method of polymerase chain reaction (PCR) for detection of group A rotavirus nucleic acid in faecal samples.About 342 nu- clcotides were amplified with them.We have tested 89 faecal samples of 19 cases rotavirus asymptomatic infec- tion of neonates by using reverse transcrip-tase-polyme- rase chain reaction (RT-PCR) and for comparision with polyacrylamide gel electrophoresis (PAGE).Of 89 fae- cal samples from the 18 neonates,54(60.1%)con- tained rotavirus RNA detected by RT-PCR,whereas only 35 (38.1%) were detected for rotavirus RNA by PAGE.the result probably close to the actual situation of the neonates shedding rotavirus.