川芎嗪对大鼠蛛网膜下腔出血后脑血管痉挛病理变化的影响

The effect of tetram ethyloyrazine on the pathological changes of cerebral vasospasm after subarachnoid hemorrhage in Rats

摘要:

目的 探讨川芎嗪对大鼠蛛网膜下腔出血(SAH)后CVS病理变化的影响。方法 健康成年SD大鼠60只随机分为四组(n=15)。①A为NS组:枕大池两次穿刺注入生理盐水 ②B为SAH+NS组 ③C为SAH+Nimotop组 ④D为SAH+TMP组。B、C、D三组均在枕大池两次注入自体动脉血制作成SAH模型后,自当天开始分别经腹腔注人生理盐水2 ml,或尼莫同0.1 mg,或川芎嗪20 mg,每日一次直到动物处死。各组动物分别在第1、4、7天分三批处死,每批5只 。结果 A组动物基底动脉组织结构正常。B组动物基底动脉在第1天时出现血管痉挛,第4天时出现血管壁增厚,均以第 7天时最为显著。C组动物血管痉挛程度有不同程度缓解,但第4天亦出现血管壁增厚,在第7天明显。 D组动物血管痉挛及血管壁增厚明显缓解,血管壁NF-кBp65, Cox2的表达与同时间段B组和C组比较明显减弱(P<0.05)。结论 川芎嗪对SAH脑血管痉挛具有一定的预防作用。

Abstract:

Objective To investigate the effect of tetram ethyloyrazine (TMP) on the pathological changes of cerebral vasospasm(CVS) after subarachoid hemorrhage(SAH) in rats.Methods 60 healthy adult SD rats were randomly divided into four groups (n=15).In group A: normal Saline was injected in the cisterna magna; in group B, C, D: autologous arterial was injected in the cisterna magna,and added NS 2ml or nimotop 0.1mg or TMP hydrochloride 20mg intraperitoneal injection every day.Five animals in each group were sacrificed and the brain samples were harvested at day 1, day 4 and day 7 respectively.Results The structure of basilar artery(BA) was normal in group A.The vasospasm of BA was found in group B at day 1 and day 4, especially at day 7.The vasospasm in group C was remarkably remitted compared with that in SAH group at the same period.The vasospasm and vsacular wall thickening and expression of NF-кBp65 and Cox2 in group D was remarkably remitted compared with that in group B and C.Conclusions TMP may has the potential ability to prevent CVS after SAH.