脑出血偏瘫大鼠瘫肢正中神经不同时期的超微结构变化

Observation on the ultra-microstructure of paralysis limb median nerves in rats with cerebral hemorrhage

摘要:

目的：观察脑出血大鼠瘫肢正中神经超微结构的变化。方法：Wistar大鼠60只，模型组及假手术各30只，通过胶原酶加肝素联合注射法建立大鼠脑出血动物模型。造模成功后第3天、14天分别分离瘫肢和健肢正中神经，活检、固定，切片、染色，观察正中神经镜下变化（光镜、电镜）。结果：无论是模型组还是假手术祖，光镜下计数大鼠正中神经有髓神经髓鞘无明显变化。同时电镜下观察正中神经超微结构未发现有髓神经轴突肿胀、未出现髓鞘脱失、间质水肿等超微结构改变。结论：脑出血偏瘫大鼠瘫肢周围神经结构无明显损害。

Abstract:

Objective: To study the changes of ultra-microstructures of paralysis limb median nerves in rat model of cerebral hemorrhage.Methods: Sixty Wistar rats were divided randomly into two groups: model and sham-operated.The cerebral hemorrhage model was prepated by an injection of collagenase plus heparin.Sampling the median nerves from both the paralysis limb and unaffected limb 3 and 14 days after cerebral hemorrhage.The nerve tissues were biopsied,fixed,sectioned and stained.The ultra-microstructures of the median nerves were observed under light and electron microscopes.Results: The myelins of median nerves were not obviously changed in both groups under a light microscope.No changes of the ultra-microstructure of median nerves were found under an electron microscope in both groups.Conclusions: The ultra-microstructures of paralysis limb median nerves were not obviously impaired in rats with cerebral hemorrhage.