MK-801在脊髓缺血中的神经保护作用

The neuroprotective effect of MK-801 on spinal cord ischemic injury

摘要:

目的 地佐环平（MK-801）对脊髓缺血中神经保护作用的研究。方法 建立新西兰兔脊髓缺血模型，利用HE染色、原位末端转移酶标记技术（TUNEL）、免疫组化、逆转录反应系统（RT-PCR）等技术检测N-甲基-D-天门冬氨酸受体（N-methyl-D-aspartate receptor，NMDAR）、诱导型一氧化氮合酶（inducible Nitric Oxide Synthase，iNOS）、半胱氨酸天冬氨酸蛋白酶3（Caspase-3）的表达水平，观察不同剂量MK-801在脊髓缺血中的神经保护作用。结果 对照组脊髓结构完全消失，低剂量组结构较完整，高剂量组缺血损害程度最轻，假手术组脊髓结构正常。NMDAR、iNOS、Caspase-3等蛋白在神经元中有明确表达。凋亡指数、NMDAR、iNOS、Caspase-3 mRNA表达水平在对照组最高，低剂量组、高剂量组，假手术组则逐渐降低，差别具有统计学意义（P<0.05）。结论 MK-801能抑制神经细胞凋亡，对脊髓缺血具有神经保护作用。

Abstract:

Objective To study the neuroprotective effect of MK-801 on spinal cord ischemic injury.Methods Establishing spinal cord ischemia model of New Zealand white rabbits.Using the HE staining, TUNEL method, immunohischemistry staining and RT-PCR to detect expression levels of NMDAR, iNOS, caspase-3 and observe the neuroprotective effect of different doses of MK-801on spinal cord ischemic injury.Results Normal structure of the spinal cord completely disappeared in the control group.Structure of low-dose group is better than the control group.Sham group showed normal spinal cord structure.Ischemic damage of high dose group ranged from the low-dose group to the sham group.NMDAR, iNOS Caspase-3 were mainly located in the cytoplasm.Apoptotic index is the highest in the control group and from low dose group, high dose group to sham group gradually decreased with statistical significance (P<0.05).NMDAR, iNOS, Caspase-3 mRNA levels gradually decreased with statistical significancance from control group, low dose group, high dose group to sham group (P<0.05).Conclusions MK-801can inhibit the neuronal apoptosis.It has the neuroprotective effect.