90K/Mac-2 BP在人脑星形细胞瘤中的表达

Expression of 90K/Mac-2 BP in human brain astrocytic tumors

摘要:

目的 分析90K/Mac-2 BP（Mac-2 binding protein）在人脑星形细胞瘤中的表达。方法 RT-PCR和WB（Western blot）检测90K蛋白在人脑星形细胞瘤以及正常脑组织中的表达。结果 RT-PCR发现90K mRNA在正常脑组织中微量表达，相对表达量为0.116±0.017；而在人脑星形细胞瘤中高表达，相对表达量为0.407±0.151，两组相比有显著差异（t=6.065，P<0.05）。低级别组（WHO Ⅰ-Ⅱ级）与高级别组（WHO Ⅲ-Ⅳ级）相对表达量分别为0.295±0.067和0.516±0.128，两组相比有显著差异（t=8.138，P<0.05）。WB发现90K蛋白在正常脑组织中微量表达，相对表达量为0.291±0.064，星形细胞瘤中90K 蛋白相对表达量为1.163±0.391，两组相比有显著差异（t=15.68，P<0.05）。低级别组与高级别组90K蛋白相对表达量分别为0.902±0.272和1.415±0.318，两组相比有显著差异（t=6.539，P<0.05）。WB结果表明90K蛋白在星形细胞瘤中的表达情况与RT-PCR结果表明90K mRNA在星形细胞瘤中的表达情况一致。结论 90K在星形细胞瘤中的表达显著升高，高级别星形细胞瘤中的表达较低级别更高，提示90K可能在星形细胞瘤的发生发展过程中发挥了重要作用，90K蛋白可能是星形细胞瘤相关抗原，在今后的免疫治疗中可能作为一种目标抗原。

Abstract:

Objective To investigate the expression of 90K mRNA and protein in human brain astrocytomas.Methods The expression of 90K mRNA in human normal brain tissue and astrocytoma tissue was detected by RT-PCR; The expression of 90K protein in human normal brain tissue and astrocytoma tissue was detected by WB.Results RT-PCR reveal that 90K mRNA was lowly expressed in normal human brain tissues (0.116±0.017).The 90K mRNA expression was significantly higher in astrocytomas (0.407±0.151) than that in normal human brain tissues (t=6.065, P<0.05), and significantly higher in high grade astrocytomas (WHO Ⅲ-Ⅳ) (0.516±0.128) than in low grade (WHOⅠ-Ⅱ) (0.295±0.067) (t=8.138, P<0.05).WB reveal that 90K protein was lowly expressed in normal human brain tissues (0.291±0.064).The 90K protein expression was significantly higher in astrocytomas (1.163±0.391) than that in normal human brain tissues (t=15.68, P<0.05), and significantly higher in high grade astrocytomas (1.415±0.318) than in low grade (0.902±0.272) (t=6.539, P<0.05).The expression of 90K mRNA was in accordance with the expression of 90K protein both in astrocytomas and normal human brain tissues.Conclusions 90K expressed positive in astrocytomas.The expression of 90K is higher in higher grade astrocytomas.90 K may contribute to occurrence and progression of astrocytomas.90K protein may be a astrocytomas-associated antigen and target antigen in immunotherapy.