失眠模型大鼠脑干γ-氨基丁酸转运体-1表达的变化

Expression of γ-aminobutyric acid transporter-1 in the brainstem of insomnia model rats

摘要:

目的 检测失眠模型大鼠脑干组织中γ-氨基丁酸转运体-1(GAT-1)mRNA及GAT-1蛋白的表达变化。方法 36只SD大鼠随机分为失眠模型组、生理盐水对照组和地西泮干预组。腹腔注射对氯苯丙氨酸(PCPA)制作大鼠失眠模型。采用RT-PCR法和Western blot法分别检测大鼠脑干组织中GAT-1 mRNA和GAT-1蛋白的表达变化。结果 与对照组相比,模型组和干预组GAT-1 mRNA及GAT-1蛋白的表达均降低(P<0.01),干预组较模型组表达更低(P<0.01)。结论 脑干GAT-1表达下调可能与失眠后机体的保护性机制有关;地西泮下调 GAT-1可能是其发挥催眠作用的机制之一。

Abstract:

Objective To study the expression of gama-aminobutyric acid transporter-1 (GAT-1) mRNA and protein in the brain stem of insomnia model rats.Methods Thirty-six SD rats were randomly divided into three groups: insomnia model, normal saline control and diazepam interference. A rat insomnia model was prepared by intraperitoneal injections of ρ-chlorophenylalanine (PCPA). The expression of GAT-1 mRNA and protein in the brainstem were detected by RT-PCR and Western blot.Results Compared with the control group, the expression of GAT-1 mRNA and protein in the model and interference groups was down-regulated (P<0.01). The interference group showed significantly lower expression of GAT-1 than the model group (P<0.01).Conclusions The down-regulation of the expression of GAT-1 may be involved in the body's protective mechanisms after insomnia. Diazepam can decrease GAT-1 expression, possibly thus providing its hypnotic function.