β淀粉样蛋白对阿尔茨海默病转基因果蝇认知功能的影响

Effect of β-amyloid proteins on cognitive functions in transgenic drosophila with Alzheimer’s disease

摘要:

目的 探讨Aβ40、Aβ42、Aβ42Arc对阿尔茨海默病（AD）转基因果蝇认知功能的影响。方法 分别建立Aβ40、Aβ42、Aβ42Arc转基因AD果蝇模型。根据实验要求将果蝇分为4组，分别是野生果蝇对照组、Aβ40组、Aβ42组、Aβ42Arc组。在整体动物水平上进行果蝇行为学研究，分别检测各组果蝇的嗅觉短期记忆能力、攀爬能力和平均寿命。结果 在巴甫洛夫嗅觉相关短期记忆测试、攀爬能力试验、平均寿命测试中，与对照组相比，3组转基因果蝇的嗅觉记忆能力、攀爬能力、平均寿命均明显降低。结论 Aβ的毒性作用会导致AD转基因果蝇认知功能下降。

Abstract:

Objective To study the effects of β-amyloid proteins Aβ40, Aβ42, and Aβ42Arc on the cognitive functions in the transgenic drosophila with Alzheimer's disease (AD).Methods The experiment was designed into four groups: normal wild-type, Aβ40, Aβ42 and Aβ42Arc transgenic drosophila. Pavlovian olfactory memory and climbing abilities and life span were measured in the four groups.Results Compared with the control group, the short-term dfactory memory and climbing abilities as well as life span were reduced in the Aβ40, Aβ42 and Aβ42Arc groups.Conclusions Aβ may result in a decrease in cognitive functions in transgenic drosophila with AD.