黄芩苷对海人酸致痫小鼠海马X染色体连锁凋亡抑制蛋白表达的影响

Effect of baicalin on expression of X-linked inhibitor of apoptosis protein in hippocampus of mice after kainic acid-induced status epilepticus

摘要:

目的 探讨黄芩苷对海人酸诱导的小鼠癫痫持续状态后海马组织X染色体连锁凋亡抑制蛋白（XIAP）表达的影响。方法 将90只ICR雄性小鼠随机分为对照组、癫痫持续状态（SE）组、黄芩苷治疗组，每组30只；采用侧脑室注入海人酸建立小鼠癫痫持续状态模型。通过苏木素－伊红（HE）染色观察黄芩苷对小鼠癫痫持续状态后海马神经细胞的形态学改变；应用免疫组化和Western blot方法检测小鼠海马组织中XIAP的表达量。结果 黄芩苷明显改善SE后小鼠海马组织的病理形态学，海马CA3区XIAP在SE后6 h起逐渐增加，12 h达高峰，24 h有所下降；与对照组比较，差异有统计学意义（P<0.01）。黄芩苷治疗组海马XIAP蛋白表达在6 h、12 h和24 h均高于SE组（P<0.05）。结论 黄芩苷对小鼠癫痫持续状态后海马神经细胞的保护作用，可能与其促进XIAP的表达有关。

Abstract:

Objective To investigate the effect of baicalin on the expression of X-linked inhibitor of apoptosis protein (XIAP) in the hippocampus of mice after kainic acid-induced status epilepticus (SE).Methods Ninety male ICR mice were randomly and equally divided into three groups: control, SE, and baicalin treatment groups. A mouse model of SE was established by injection of 0.1 μg/5 μl kainic acid into the lateral ventricle. HE staining was used to observe the pathological changes in hippocampal CA1 and CA3 regions after SE; the expression of XIAP was measured by immunohistochemistry and Western blot.Results Baicalin significantly reduced the pathological changes in the hippocampus of mice after SE. In the SE group, the expression of XIAP in hippocampal CA1 and CA3 regions increased gradually since 6 hrs after SE, reached the peak level at 12 hrs, and decreased at 24 hrs; the expression of XIAP was significantly higher in the SE group than in the control group at the three time points (P<0.01); compared with the SE group, the baicalin treatment group had significantly higher expression of XIAP in hippocampal CA1 and CA3 regions at 6, 12, and 24 hrs (P<0.05).Conclusions Baicalin has a protective effect on the hippocampal neurons in mice after SE, which might be associated with the up-regulated expression of XIAP.