PPARγ基因C1431T多态性及其与脑出血的关系

Relationship between PPARγ gene C1431T single nucleotide polymorphism and cerebral hemorrhage

摘要:

目的 探讨过氧化物酶体增殖物激活受体γ(PPARγ)基因C1431T多态性在人群中的分布及其与脑出血的关系。方法 用聚合酶链反应-限制性片段长度多态性分析法检测PPARγ基因C1431T多态性在脑出血组和正常对照组的分布情况。结果 PPARγC1431T基因型CC、CT+TT在脑出血患者组的分布频率分别为43.8%、56.2%,C等位基因频率为70.1%,T等位基因频率为29.9%。CC、CT+TT基因型在正常对照组中的分布频率分别为62.1%、37.9%,C等位基因频率为79.8%,T等位基因频率为20.2%。脑出血组PPARγ基因1431 T/X基因型频率(P=0.000)和T等位基因频率(P=0.000)均显著高于对照组,差异有统计学意义。结论 PPARγ基因C1431T多态性可能与脑出血存在关联,T等位基因可能与脑出血发病率呈正相关。

Abstract:

Objective To study the distribution of peroxisome proliferators-activated receptor gamma (PPARγ) gene C1431T single nucleotide polymorphism (SNP) in a Chinese population and its relationship with cerebral hemorrhage (CH).Methods The distribution of PPARγ gene SNP in the CH group and the control group was determined by polymerase chain reaction-restriction fragment length polymorphism.Results In the CH group, the frequencies of PPARγ gene C1431T genotypes, CC and CT+TT, were 43.8% and 56.2%, respectively, while the frequencies of the C allele and the T allele were 70.1% and 29.9%, respectively. In the control group, the frequencies of the genotypes, CC and CT+TT, were 62.1℅ and 37.9℅, respectively, while the frequencies of the C allele and the T allele were 79.8% and 20.2%, respectively. Therefore, the frequencies of PPARγ gene 1431 T/X genotype and the T allele were significantly higher in the CH group than in the control group (P=0.000; P=0.000).Conclusions The PPARγ gene C1431T SNP may be associated with CH, and the C allele may be positively associated with the incidence of CH.