MGMT、Caspase-3蛋白在胶质瘤中的表达及预后分析

MGMT and caspase-3 expression and their role in the survival prognosis of glioma patients

摘要:

目的 探讨MGMT、Caspase-3蛋白表达水平与胶质瘤患者生存预后的关系。方法 运用免疫组织化学方法检测75例不同病理级别胶质瘤组织和10例正常脑组织MGMT、Caspase-3表达情况，分析两者与胶质瘤患者临床病理特征及预后的相关性。结果 MGMT、Caspase-3在胶质瘤中的表达均高于正常脑组织，差异有统计学意义。MGMT、Caspase-3表达水平与患者的年龄、性别、术前KPS评分、病理级别无关联。两者表达情况与恶性胶质瘤患者的生存期关系用Kaplan-Meier生存曲线表示，结果表明MGMT表达水平与患者预后呈负相关（P<0.05），Caspase-3表达水平与患者预后无相关性（P>0.05）。MGMT和Caspase-3两者表达呈负相关（r=-0.230，P=0.047）。结论 MGMT表达与胶质瘤预后有关，可作为胶质瘤预后的生物学指标之一，Caspase-3表达与胶质瘤预后无关，但与MGMT有关联。

Abstract:

Objective To determine the relationship between MGMT and caspase-3 expression levels and the survival prognosis of glioma patients.Methods MGMT and caspase-3 expression in 75 cases of glioma tissues (of different pathological grades) and 10 cases of normal brain tissues were measured by immunohistochemistry, and their correlation with the clinicopathological characteristics and survival prognosis of patients was analyzed.Results MGMT and caspase-3 expression was significantly higher in glioma tissues than in normal brain tissues (P<0.05). The levels of MGMT and caspase-3 expression were not correlated with the patients' age, sex, preoperative Karnofsky Performance Scale score, and pathological grade of glioma. The Kaplan-Meier survival curve indicated that MGMT expression in gliomas was negatively correlated with survival prognosis (P<0.05), while caspase-3 expression was not correlated with prognosis (P>0.05). MGMT expression was negatively correlated with caspase-3 expression (r=-0.230, P=0.047).Conclusions MGMT expression is associated with the prognosis of glioma and can be used as a prognostic biomarker for glioma. On the other hand, caspase-3 expression is not associated with the prognosis of glioma patients, but is negatively correlated with MGMT.