甲状腺激素受体β 1在非典型脑膜瘤的表达及其作用研究

Expression of thyroid hormone receptor β1 and its role in atypical meningioma

摘要:

目的 研究甲状腺激素受体β1（TRβ1）和非典型脑膜瘤的关系及其作用。方法 通过real-timePCR和免疫组化分别对良性脑膜瘤和非典型脑膜瘤中的TRβ1进行检测；同时我们在体外进行原代脑膜瘤细胞培养，并通过MTT检测TRβ1对非典型脑膜瘤细胞的影响。结果 TRβ1mRNA在非典型脑膜瘤中的表达低于良性脑膜瘤，没有统计学意义（P>0.05）；但TRβ1阳性细胞在非典型脑膜瘤为平均40.75个/mm²低于良性脑膜瘤93个/mm²，有统计学意义（P<0.05）。而在体外实验中，T3干预可有效抑制非典型脑膜瘤细胞的增殖，在浓度为20 ng/ml时MTT OD值由对照组0.24±0.028下降到0.17±0.008，抑制作用明显，具有统计学意义（P<0.05），而在siRNA沉默TRβ1蛋白的表达后，可有效减弱T3对细胞的抑制作用。结论 TRβ1在非典型脑膜瘤上的表达下降，在被其配体T3激活后可有效抑制非典型脑膜瘤细胞增殖。提示TRβ1可能在非典型脑膜瘤的发生及生长过程中扮演着重要角色。

Abstract:

Objective To investigate the relationship between thyroid hormone receptor β1 (TRβ1) and atypical meningioma, and to investigate the role of TRβ1 in atypical meningioma. Methods Real-time PCR and immunohistochemistry were used to measure the mRNA and protein expression of TRβ1 in benign meningioma and atypical meningioma. In addition, primary meningioma cells were cultured in vitro, and MTT assay was used to investigate the effect of TRβ1 on atypical meningioma cells. Results The mRNA expression of TRβ1 in atypical meningioma was lower than that in benign meningioma (P>0.05). Immunohistochemistry results showed that the mean number of TRβ1+ cells in atypical meningioma was significantly lower than that in benign meningioma (40.75 cells/mm² vs 93 cells/mm², P<0.05). In the assay in vitro, T3 effectively inhibited the proliferation of atypical meningioma cells, reducing MTT absorbance from 0.24±0.028 in the control group to 0.17±0.008 in the treated group at a concentration of 20 ng/ml (P<0.05), which suggested that T3 had a marked inhibitory effect on the proliferation. When the expression of TRβ1 protein was down-regulated by small interfering RNA, the inhibitory effect of T3 on atypical meningioma cells was effectively attenuated. Conclusions The expression of TRβ1 in atypical meningioma is lower than that in benign meningioma. And TRβ1 can effectively inhibit the proliferation of atypical meningioma cells after being activated by T3. This suggests that TRβ1 may play an important role in the development and progression of atypical meningioma.