CUX-1在胶质瘤中的表达及其对临床病理和预后的意义

Expression of CUX-1 in glioma and its significance for the clinicopathological features and prognosis of patients

摘要:

目的 探究转录因子CUX-1在胶质瘤中的表达及其对临床病理和患者预后的意义。方法 在TCGA数据库中对CUX-1在肿瘤和正常脑组织中的差异性表达及其对患者总体生存期的影响进行生物信息学分析。运用蛋白印迹、免疫组化实验检测CUX-1在不同级别胶质瘤中的表达，同时分析其与临床病理指标及患者预后的关系。结果 生信分析结果表明CUX-1在胶质瘤中表达显著上调，且CUX-1高表达明显缩短患者生存期。蛋白印迹、免疫组化结果证明CUX-1随肿瘤WHO分级的升高而上调（P<0.05），且CUX-1与胶质瘤WHO分级及增殖指标Ki67、P53mut显著相关（P<0.05）。Kaplan-Meir分析示CUX-1低表达患者生存期显著长于高表达者（P<0.01）。结论 CUX-1在胶质瘤中显著上调，且与肿瘤增殖密切相关，同时CUX-1高表达提示患者预后较差。综上，CUX-1有望作为胶质瘤预后的分子标志物和治疗的潜在靶点。

Abstract:

Objective To investigate the expression of the transcription factor CUX-1 in glioma and its significance for the clinicopathological parameters and prognosis of patients.Methods TCGA database was used to perform a bioinformatic analysis on the differential expression of CUX-1 in tumor and normal brain tissue and its effect on the overall survival (OS) of patients. Western blot and immunohistochemistry were used to determine CUX-1 expression in different grades of gliomas and analyze its effects on the clinicopathological parameters and prognosis of patients.Results The bioinformatic analysis indicated that CUX-1 expression was significantly increased in glioma and high CUX-1 expression significantly shortened the survival of patients. The results of Western blot and immunohistochemistry indicated that CUX-1 expression was up-regulated with increasing WHO grade of tumor (P<0.05) and was significantly correlated with the WHO grade of glioma and the levels of proliferation indices (Ki67 and P53mut) (P<0.05). The Kaplan-Meier analysis suggested that patients with low CUX-1 expression had significantly longer overall survival than those with high CUX-1 expression (P<0.01).Conclusions CUX-1 expression is significantly up-regulated in glioma and is closely associated with tumor proliferation. Meanwhile, high CUX-1 expression suggests poor prognosis of the patient. In summary, CUX-1 holds promise as a prognostic molecular biomarker and potential therapeutic target for glioma.