CUX-1和XRCC3在胶质瘤中的表达及其与预后的关系

Expression of homeobox protein cut-like 1 and X-ray repair cross-complementing group 3 in glioma and its association with prognosis

摘要:

目的 探讨同源框CUT样蛋白1（CUX-1）及X射线交错互补修复基因3（XRCC3）在组织中的表达水平，及与胶质瘤患者病理指标及预后的关系。方法 采用免疫组织化学染色及蛋白质印迹法检测66例胶质瘤组织以及10例正常脑组织CUX-1及XRCC3的表达水平，分析它们之间的关系及其与患者临床病理指标和预后的关系。结果 CUX-1和XRCC3表达水平随肿瘤WHO分级的升高而上调（P<0.01），且两者的表达与胶质瘤WHO分级及增殖指标Ki67、P53mut相关（P<0.01）。CUX-1与XRCC3之间的表达呈正相关（r_s=0.773，P=0.006）。Kaplan-Meier生存曲线表明CUX-1及XRCC3高表达患者生存时间缩短（P<0.05）。结论 CUX-1及XRCC3两者之间的表达呈正相关，并在胶质瘤中表达上调，且与不良预后相关。

Abstract:

Objective To investigate the expression levels of homeobox protein cut-like 1 (CUX-1) and X-ray repair cross-complementing group 3 (XRCC3) in glioma and their association with clinicopathological parameters and prognosis.Methods Immunohistochemistry and Western blotting were used to measure the expression levels of CUX-1 and XRCC3 in 66 glioma tissue samples and 10 normal brain tissue samples, and their association with clinicopathological parameters and prognosis was analyzed.Results The expression levels of CUX-1 and XRCC3 increased with the increase in glioma WHO grade (P<0.01) and were correlated with glioma WHO grade and the proliferation indices Ki67 and P53mut (P<0.01). The expression of CUX-1 was positively correlated with that of XRCC3 (r_s=0.773, P=0.006). The Kaplan-Meier survival curve analysis showed that patients with high expression of CUX-1 and XRCC3 had a significantly shortened overall survival time than those with low expression (P<0.05).Conclusions The expression of CUX-1 is positively correlated with that of XRCC3, and both of them are upregulated in glioma, which is associated with poor prognosis.