黏附G蛋白偶联受体L3在脑胶质瘤中的表达及预后分析

Expression of adhesion G protein-coupled receptor L3 in glioma and its prognostic significance

摘要:

目的 探讨黏附G蛋白偶联受体L3（ADGRL3）在胶质瘤中的表达及与患者预后的关系。方法 通过生物信息学数据库和分析工具，首先分析ADGRL3在泛癌中的表达，进而分析ADGRL3在不同级别胶质瘤中的表达水平，比较ADGRL3在不同病理特征的胶质瘤间表达差异，并探讨其与胶质瘤患者预后的关系；随后采用DAVID数据库对STRING和GeneMANIA数据库筛选的相互作用基因与蛋白进行GO富集分析；最后采用TIMER数据库，对ADGRL3进行免疫浸润分析。结果 ADGRL3在多种肿瘤中高表达，且在脑低级别胶质瘤与胶质母细胞瘤的表达水平高于其他肿瘤，其表达水平随着脑胶质瘤级别的升高而降低。ADGRL3的表达水平与多个临床病理指标相关。在脑低级别胶质瘤中，高表达ADGRL3的患者比低表达的患者预后好（P＜0.05）。然而，在胶质母细胞瘤中ADGRL3的表达水平高低与患者预后没有相关性（P＞0.05）。STRING、GeneMANIA、DAVID数据库分析发现ADGRL3的互作基因与蛋白富集于信号转导、蛋白质异源二聚体活化、神经元投射等过程。在脑肿瘤中ADGRL3与CD8⁺ T细胞浸润相关。结论 ADGRL3能够影响胶质瘤的发生、发展和预后，可以作为胶质瘤患者预后的生物标志物。

Abstract:

Objective To investigate the expression of adhesion G protein-coupled receptor L3 （ADGRL3） in glioma and its association with the prognosis of glioma patients.Methods Bioinformatics database and tools were used to analyze the expression of ADGRL3 in pan-cancer and compare the level of ADGRL3 between different grades of glioma and between glioma patients with different pathological features， and the association of ADGRL3 with the prognosis of glioma patients was analyzed. DAVID database was used to perform gene ontology enrichment analysis of the interacting genes and proteins identified by SRTING and GeneMANIA databases. Finally TIMER database was used to perform immune infiltration analysis of ADGRL3.Results ADGRL3 was highly expressed in various tumors， with a significantly higher expression level in low-grade glioma and glioblastoma compared with other tumors， and its expression level decreased with the increase in the grade of glioma. The expression of ADGRL3 was correlated with multiple clinicopathological parameters. As for low-grade glioma， the patients with high ADGRL3 expression had a better prognosis than those with low expression （P <0.05）； however in glioblastoma， the expression level of ADGRL3 was not significantly associated with the prognosis of patients （P >0.05）. The analysis of SRTING， GeneMANIA， and DAVID databases showed that the interacting genes and proteins of ADGRL3 were mainly enriched in the processes such as signal transduction， protein homodimerization activity， and neuron projection. ADGRL3 was associated with the infiltration of CD8⁺ T cells in brain tumor.Conclusion ADGRL3 can affect the development， progression， and prognosis of glioma and may be used as a biomarker for the prognosis of glioma patients. ［Journal of International Neurology and Neurosurgery, 2024, 51(5): 75-82］